Preparing tissue for microscopic observation for estimating volume and/or cross-sectional area.

Use systematic random sampling through the entire region of interest.

Preferential sections may be used.

Thin sections may be used.

If you are estimating the area of a homogeneous cross-section or the volume of a homogeneous region, use the Cavalieri/point-counting probe. If you are estimating the percentage by volume or area of a component, use the Area Fraction Fractionator probe.