THE NUMBER-WEIGHTED VOLUME OF A CELL ESTIMATED WITH THE NUCLEATOR PROBE
Section thickness required: thick sections if using a disector for number weighted sampling
Orientation of tissue required: isotropic or vertical sections
The optical disector is used to pick the cell without bias in a number weighted manner.
The example above is from an isotropic section.
This example is from a vertical section, the vertical direction (black arrow) must be indicated before the nucleator rays are placed.
____________________________________________________________________
Sponsored by MBF Bioscience
developers of Stereo Investigator, the world’s most cited stereology system
